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| tyc122ccvip类型 | 货号 | tyc122ccvip描述 | 应用场景 |
|---|---|---|---|
| 转染试剂 | TF-1157-11 | BPfectin Transfection Reagent | 高效转染试剂，用于将质粒DNA递送至哺乳动物细胞（如HEK293），提升转染效率 |
| 细胞培养基 | CM-1156-11 | CD 293 TGE Medium, 1X, Liquid | 专为HEK293细胞高密度悬浮培养开发的无血清培养基，支持瞬时基因表达（TGE）技术，显著提升蛋白产量 |
| 表达增强试剂 | EXP-711-11 | 293 Expression MAX-1 | 转染后添加的增效试剂，通过调控细胞代谢与表达环境，显著提升目标蛋白的表达量 |
| 细胞培养补料 | CF-1116-12 | CD Feed X Supplement, Liquid | 为培养中后期补充营养，维持细胞活力并延长培养周期，提升产物产量 |
| 抗结团添加剂 | AC-1112-11 | Anti-Clumping Additive | 防止悬浮培养中细胞聚集，改善细胞分散性，提升培养均一性和收获效率 |
通过整合高效转染（BPfectin Transfection）、快速细胞扩增（CD 293 TGE Medium）、强化表达（293 Expression）、精准营养调控（Feed X Supplement）及防聚集工艺优化（Anti-Clumping Additive），本系列tyc122ccvip构建了无缝衔接的工作流程。 其显著成果在于：降低批次间差异、提高目标产物得率，从而驱动科研与工业生产的成本降低与效率提升。
CD 293 TGE Medium (Cat. No. CM-1156-11 ) delivers over 200% cell growth compared to competitive alternatives.
Serum free adapted HEK293 cell line was employed in transient expression experiment. Compared with the transfection without 293 Expression MAX-1, the expression titer of human IgG1 Fc protein was over tripled fold increased when the 293 Expression MAX-1 reagent (Cat. No. EXP-711-11 ) was applied (40uL reagent per 100 ug TGE plasmid).
HEK293F, Clumped Cells (Added 0 h)
Cell Image taken by Cell Counter (Luna I), with significant cell clumping arising from suspension culture using Anti-Clumping Additive (Cat. No. AC-1112-11), at 0 h.
HEK293F, Given Anti-Clumping Additive (Added 24 h)
Cell Image taken by Cell Counter (Luna I), with very low level of cell clumping arising from suspension culture using Anti-Clumping Additive (Cat. No. AC-1112-11), at 24 h.
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